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Caspase 8 antibody [EPR162]

Anti-Caspase 8 antibody [EPR162] used in IHC (Paraffin sections) (IHC-P). GTX63075
Anti-Caspase 8 antibody [EPR162] used in Western Blot (WB). GTX63075
Anti-Caspase 8 antibody [EPR162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX63075

Cat. No. GTX63075

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EPR162

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Caspase 8 products

APPLICATION

Application Note

Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000 - 1:10000. For IHC-P: Use at a dilution of 1:100 - 1:250. For ICC/IF: Use at a dilution of 1:100 - 1:250. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 0.05% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to human Caspase-8 aa 200-300 (internal sequence).

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caspase 8 antibody [EPR162] used in IHC (Paraffin sections) (IHC-P). GTX63075

GTX63075 IHC-P Image

IHC-P analysis of human hepatocellular carcinoma using Caspase 8 antibody [EPR162] at a dilution of 1:100.

Anti-Caspase 8 antibody [EPR162] used in Western Blot (WB). GTX63075

GTX63075 WB Image

WB analysis of lysates from (1) etoposide-treated Jurkat, (2) untreated Jurkat, (3) staurosporine-treated HeLa and (4) untreated HeLa cells (10 μg per lane) using Caspase 8 antibody [EPR162] at a dilution of 1:1,000.

Anti-Caspase 8 antibody [EPR162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX63075

GTX63075 ICC/IF Image

ICC/IF analysis of HeLa cells using Caspase 8 antibody [EPR162] at a dilution of 1:100.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399