GeneTex
United States (US)

Caspase 8 antibody [FLICE 4-1-20]

GTX30244_1376_WB_18121410_323.jpg

Cat No. GTX30244

Host Mouse
Clonality Monoclonal
Clone Name FLICE 4-1-20
Isotype IgG1
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human Caspase 8 [UniProt# Q14790]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALPS2B antibody , CAP4 antibody , Casp-8 antibody , FLICE antibody , MACH antibody , MCH5 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30244_1376_WB_18121410_323.jpg

GTX30244 WB Image

WB analysis of HEK293 cell lysate using GTX30244 Caspase 8 antibody [FLICE 4-1-20].
Lanes 1 : inactive Caspase 8
Lane 2 : active Caspase 8
Dilution : 1:500