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Caspase 8 antibody [GT1152]

Anti-Caspase 8 antibody [GT1152] used in Western Blot (WB). GTX01015
Anti-Caspase 8 antibody [GT1152] used in Western Blot (WB). GTX01015

Cat No. GTX01015

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1152

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifμge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Caspase-8

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein sμggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, althoμgh not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caspase 8 antibody [GT1152] used in Western Blot (WB). GTX01015

GTX01015 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody [GT1152] (GTX01015) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 10894161

Anti-Caspase 8 antibody [GT1152] used in Western Blot (WB). GTX01015

GTX01015 WB Image

WB analysis of Wild-type (WT) and Caspase 8 knockout (KO) HeLa cell extracts using GTX01015 Caspase 8 antibody [GT1152].
Dilution : 1:1000
Loading : 25μg

REFERENCE

There are currently no references for Caspase 8 antibody [GT1152] (GTX01015). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369