Caspase 8 antibody

Cat No. GTX10450

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human

See all Caspase 8 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1,000

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Raji

Specificity/Sensitivity

By immunoblotting, the antibody reacts with caspase 8a (55 kDa) and caspase 8b (54 kDa) and weakly cross-reacts with MACHβ3/MACHβ4 isoforms (30-32 kDa).

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 209-224 of the human caspase 8a/MACHα1 precursor, conjugated to KLH. The sequence contains the N-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (caspase 8b/MACHα2, MACHβ3, MACHβ4) and highly conserved (>85%) in mouse caspase 8.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Caspase 8,Alps2B,Cap4,Casp-8,Flice,Mach,Mch5,Casp8

Background

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.