Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1,000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji
Product Note
By immunoblotting, the antibody reacts with caspase 8a (55 kDa) and caspase 8b (54 kDa) and weakly cross-reacts with MACHβ3/MACHβ4 isoforms (30-32 kDa).
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic peptide corresponding to amino acids 209-224 of the human caspase 8a/MACHα1 precursor, conjugated to KLH. The sequence contains the N-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (caspase 8b/MACHα2, MACHβ3, MACHβ4) and highly conserved (>85%) in mouse caspase 8.
Purification
IgG fraction
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
Cellular Localization
Cytoplasm
Background
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase_x0013_9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.
Database
Research Area