*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The sequence used as the immunogen corresponds to the C-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (MACHα2, MACHα3, MACHβ) and has limited homology (approx. 50%) to mouse caspase 8. By immunoblotting, the antibody reacts with caspase 8a and caspase 8b.
0.01M PBS pH7.4, 15 mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
synthetic peptide of the human caspase 8a/MACHα1 precursor (amino acids 362-374 with N-terminal added lysine), conjugated to KLH.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse.