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Caspase 8 antibody

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723
Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723
Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723
Anti-Caspase 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110723
Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723
Anti-Caspase 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110723
Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723
Anti-Caspase 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110723
Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723
Anti-Caspase 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110723

Cat. No. GTX110723

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036448

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723

GTX110723 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723

GTX110723 WB Image

Wild-type (WT) and CASP8 (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723

GTX110723 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110723

GTX110723 IHC-P Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723

GTX110723 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Caspase 8 antibody used in IHC (Paraffin sections) (IHC-P). GTX110723

GTX110723 IHC-P Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723

GTX110723 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110723

GTX110723 ICC/IF Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Caspase 8 antibody used in Western Blot (WB). GTX110723

GTX110723 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110723

GTX110723 ICC/IF Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: Caspase 8 protein stained by Caspase 8 antibody (GTX110723) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

REVIEW

Caspase 8 antibody Cat. No. GTX110723

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2016.PMID: 27863490
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2020.PMID: 32635653
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2018. PMID: 30297634
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2018. PMID: 30297634
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169