Caspase 8 antibody

Cat. No. GTX110723

Reference 18

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Cat, Dog
Package	100 μl ($419), 25 μl ($169)

See all Caspase 8 products

View product citations for antibody GTX110723 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036448

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]

Database

Gene ID: 841 CASP8
UniProt: Q14790 CASP8

Research Area

DATA IMAGES

GTX110723 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110723 WB Image

Wild-type (WT) and CASP8 (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110723 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX110723 IHC-P Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110723 WB Image

GTX110723 ICC/IF Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX110723 ICC/IF Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: Caspase 8 protein stained by Caspase 8 antibody (GTX110723) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX110723 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (GTX110723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110723 IHC-P Image

Caspase 8 antibody detects Caspase 8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Caspase 8 stained by Caspase 8 antibody (GTX110723) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min