GeneTex
United States (US)

Caspase 8 antibody

GTX31284_888_IHC-P_20180221_18121410_735.jpg
GTX31284_887_IHC-P_20180221_18121410_932.jpg
GTX31284_93_ICC-IF_20180221_18121410_881.jpg
GTX31284_94_ICC-IF_20180221_18121410_782.jpg
GTX31284_2487_WB_20180221_18121410_129.jpg
GTX31284_2486_WB_20180221_18121410_964.jpg

Cat No. GTX31284

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
ICC/IF 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Lysate(GTX27921) , Mouse Spleen Tissue Lysate(GTX27937)

Specificity/Sensitivity

Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 8,Alps2B,Cap4,Casp-8,Flice,Mach,Mch5,Casp8

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31284_888_IHC-P_20180221_18121410_735.jpg

GTX31284 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31284 Caspase 8 antibody.
Working concentration : 20 μg/ml

GTX31284_887_IHC-P_20180221_18121410_932.jpg

GTX31284 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31284 Caspase 8 antibody.
Working concentration : 5 μg/ml

GTX31284_93_ICC-IF_20180221_18121410_881.jpg

GTX31284 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31284 Caspase 8 antibody.
Working concentration : 2 μg/ml

GTX31284_94_ICC-IF_20180221_18121410_782.jpg

GTX31284 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31284 Caspase 8 antibody.
Working concentration : 20 μg/ml

GTX31284_2487_WB_20180221_18121410_129.jpg

GTX31284 WB Image

WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31284 Caspase 8 antibody.
Working concentration : 1 μg/ml

GTX31284_2486_WB_20180221_18121410_964.jpg

GTX31284 WB Image

WB analysis of (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate using GTX31284 Caspase 8 antibody.
Working concentration : 0.5 μg/ml

Package List Price ($)
$ 329