APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 2 μg/mL |
| 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Lysate(GTX27921) , Mouse Spleen Tissue Lysate(GTX27937)
Product Note
Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31284 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31284 Caspase 8 antibody.
Working concentration : 20 μg/ml
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GTX31284 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX31284 Caspase 8 antibody.
Working concentration : 2 μg/ml
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GTX31284 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31284 Caspase 8 antibody.
Working concentration : 5 μg/ml
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GTX31284 WB Image
WB analysis of Jurkat cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31284 Caspase 8 antibody.
Working concentration : 1 μg/ml
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GTX31284 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX31284 Caspase 8 antibody.
Working concentration : 20 μg/ml
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GTX31284 WB Image
WB analysis of (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate using GTX31284 Caspase 8 antibody.
Working concentration : 0.5 μg/ml
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REFERENCE
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REVIEW
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