For IHC-P: Use at a dilution of 1:1,000. For IP: Use at a dilution of 1:2,000. For WB: Use at a diltuion of 1:500. Optimal dilutions/concentrations should be determined by the researcher.
Jurkat Cells, Stomach
It is recommended for detection of the p18 subunit and precursor of caspase-8 of mouse, rat and human origin.
10mM Phosphate-buffered saline containing 0.2% BSA and 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The epitope corresponding to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin.
Protein A affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Caspase 8 , Alps2B , Cap4 , Casp-8 , Flice , Mach , Mch5 , Casp8
Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the proform of the Caspase 8 protein. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through ""self-cleavage"". Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. Reacts with Jurkat cells and Tonsil.