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Caspase 9 (active) antibody

Anti-Caspase 9 (active) antibody used in Western Blot (WB). GTX22324

Cat No. GTX22324

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-4 μg/ml
ICC/IF Assay dependent
IP 10-20 μg/ml
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes only the cleaved caspase 9 (37 kDa). It does not recognize full-length caspase 9 or any other caspase.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 9 , AI115399 , APAF-3 , AW493809 , CASP-9 , Caspase-9 , ICE-LAP6 , Mch6

Background

This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

Database

Research Area

DATA IMAGES

Anti-Caspase 9 (active) antibody used in Western Blot (WB). GTX22324

GTX22324 WB Image

WB analysis of (1) untreated and (2) Etoposide treated Jurkat cell lysate using GTX22324 Caspase 9 (active) antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319