GeneTex
United States (US)

Caspase 9 antibody [3-20]

GTX34168_20170112_IP_18121410_945.jpg
GTX34168_20170112_WB_18121410_934.jpg
GTX34168_20170112_WB_1_18121410_403.jpg

Cat No. GTX34168

Host Mouse
Clonality Monoclonal
Clone Name 3-20
Isotype IgG1
Application WB, IP
Reactivity Human, Mouse, Rat, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Specificity/Sensitivity

The antibody detects endogenous Caspase 9 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APAF3 Antibody , CASPASE9c Antibody , caspase 9 Antibody , MCH6 Antibody , CASP9 Antibody , ICE LAP6 Antibody , PPP1R56 Antibody

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
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Database

Research Area

DATA IMAGES
GTX34168_20170112_IP_18121410_945.jpg

GTX34168 IP Image

Immunoprecipitation (IP) analysis: 1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200

GTX34168_20170112_WB_18121410_934.jpg

GTX34168 WB Image

Western Blot (WB) analysis of HeLa, diluted at 1) 1:2000, 2) 1:5000.

GTX34168_20170112_WB_1_18121410_403.jpg

GTX34168 WB Image

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000.