Caspase 9 antibody [3-20]

Cat. No. GTX34168

Host	Mouse
Clonality	Monoclonal
Clone Name	3-20
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX34168 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:5000
IHC-P	Assay dependent
IP	1:200

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APAF3,CASP9,ICELAP6,MCH6,PPP1R56,caspase 9,Caspase 9

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Gene ID: 842 CASP9
UniProt: P55211 CASP9

Research Area

DATA IMAGES

GTX34168 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 IHC-P Image

IHC-P analysis of rat lung tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 IHC-P Image

IHC-P analysis of human lung tissue using GTX34168 Caspase 9 antibody [3-20]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34168 WB Image

WB analysis of HeLa cell lysate using GTX34168 Caspase 9 antibody [3-20].

GTX34168 WB IP Image

IP analysis of HeLa cell lysate using GTX34168 Caspase 9 antibody [3-20].
IP reaction : 1:500
IB Dilution : 1:2000 (Lane 1), 1:5000 (Lane 2)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Caspase 9 antibody [3-20]

Cat. No. GTX34168

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX34168 IHC-P Image

GTX34168 IHC-P Image

GTX34168 IHC-P Image

GTX34168 IHC-P Image

GTX34168 IHC-P Image

GTX34168 IHC-P Image

GTX34168 IHC-P Image

GTX34168 WB Image

GTX34168 WB IP Image

REFERENCE

REVIEW

Product

Research

Support

Company

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