GeneTex
United States (US)

Caspase 9 antibody [LAP6 96-2-22]

GTX30245_1378_WB_18121410_830.jpg
GTX30245_1377_WB_18121410_175.jpg
GTX30245_200_ICC-IF_18121410_517.jpg

Cat No. GTX30245

Host Mouse
Clonality Monoclonal
Clone Name LAP6 96-2-22
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:250
IHC-P 1:50 - 1:250
Not tested in other applications.

Calculated MW

46 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APAF-3 antibody , APAF3 antibody , ICE-LAP6 antibody , MCH6 antibody , PPP1R56 antibody

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30245_1378_WB_18121410_830.jpg

GTX30245 WB Image

WB analysis of Jurkat (A) and HeLa (B) cell lysate using GTX30245 Caspase 9 antibody [LAP6 96-2-22].
Dilution : 1 : 500

GTX30245_1377_WB_18121410_175.jpg

GTX30245 WB Image

WB analysis of active 293 (lane 2) and inactive 293 cell lysate(lane 1) using GTX30245 Caspase 9 antibody [LAP6 96-2-22].

GTX30245_200_ICC-IF_18121410_517.jpg

GTX30245 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30245 Caspase 9 antibody [LAP6 96-2-22].
Green : primary antibody
Red : Tubulin
Blue : DAPI

Package List Price ($)
$ 339