GeneTex

  • Country / Location Selection

United States (US)

Caspase 9 antibody [N2C3]

Orthogonal
Anti-Caspase 9 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

Cat. No. GTX112888

Reference 28
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Caspase 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949838

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 9 , APAF-3 , APAF3 , ICE-LAP6 , MCH6 , PPP1R56

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 9 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX112888

GTX112888 IHC-P Image

Caspase 9 antibody [N2C3] detects Caspase 9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Caspase 9 stained by Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody (GTX112888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody [N2C3] (GTX112888) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2013. PMID: 24359494

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal Mediators Inflamm in 2015. PMID: 25814788

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2020.PMID: 32635653

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 28467781

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 26716417

Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888

GTX112888 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 26716417

REFERENCE

Application Reference

Chu YT et al. Toxics 2023; 11 (3) Hypoxia-Induced Kidney Injury in Newborn Rats.
Application : WB
Reactivity : Rat
Yi-Chun Chao et al. Int J Mol Sci 2021; 22 (11) Melatonin Downregulates PD-L1 Expression and Modulates Tumor Immunity in KRAS-Mutant Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human, Mouse
Lam HYP et al. PLoS Negl Trop Dis 2021; 15 (6) : e0009554 Ameliorative effects of Schisandrin B on Schistosoma mansoni-induced hepatic fibrosis in vivo.
Application : WB
Reactivity : Mouse
Lam HYP et al. Parasitol Int 2021; 80 : 102231 Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain.
Application : WB
Lam HYP et al. Biomolecules 2020; 10 (7) Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice.
Show More +
Submit a Reference

REVIEW

Caspase 9 antibody [N2C3] Cat. No. GTX112888

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Date : Anonymous submitted on 16-May-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells with/without drug treatment
Amount used : 20μg
Blocking : Bullet Blocking One For Western Blotting
Primary Antibody : 1:3000, 4°C, 16Hr
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cell lysates
Blocking : HyBlock 1min Blocking Buffer, 25°C, 0.01Hr
Primary Antibody : 1:1000, 15°C, 4Hr
Anti-Caspase 9 antibody [N2C3] used in Western Blot (WB). GTX112888
Date : Anonymous submitted on 17-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : NTUB1 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Submit a Review
Application Reference
Chu YT et al. Toxics 2023; 11 (3) Hypoxia-Induced Kidney Injury in Newborn Rats.
Application : WB
Reactivity : Rat
Yi-Chun Chao et al. Int J Mol Sci 2021; 22 (11) Melatonin Downregulates PD-L1 Expression and Modulates Tumor Immunity in KRAS-Mutant Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human, Mouse
Lam HYP et al. PLoS Negl Trop Dis 2021; 15 (6):e0009554 Ameliorative effects of Schisandrin B on Schistosoma mansoni-induced hepatic fibrosis in vivo.
Application : WB
Reactivity : Mouse
Lam HYP et al. Parasitol Int 2021; 80:102231 Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain.
Application : WB
Lam HYP et al. Biomolecules 2020; 10 (7) Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice.
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Lee CW et al. Nutrients 2019; 11 (9) Djulis (Chenopodium Formosanum) Prevents Colon Carcinogenesis via Regulating Antioxidative and Apoptotic Pathways in Rats.
Application : WB
Reactivity : Rat
Tsai SL et al. Protein Expr Purif 2019; 160:73-83 Cloning, expression, and purification of the recombinant pro-apoptotic dominant-negative survivin T34A-C84A protein in Escherichia coli.
Application : WB
Reactivity : Human
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
Application : WB
Reactivity : Human
Ho Y et al. Food Chem Toxicol 2018; 120:346-355 Resveratrol inhibits human leiomyoma cell proliferation via crosstalk between integrin αvβ3 and IGF-1R.
Application : WB
Reactivity : Human
Kim SH et al. Biomedicine & Pharmacotherapy 2018; 99:997-1008 Teroxirone suppresses growth and motility of human hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Gunes-Bayir A et al. Mol Cell Biochem 2018; (Epub) Effects of carvacrol on human fibroblast (WS-1) and gastric adenocarcinoma (AGS) cells in vitro and on Wistar rats in vivo.
Application : IHC-P
Reactivity : Rat
Tan G et al. Environ. Toxicol. Pharmacol. 2018; 57:175-180 Myricetin enhances on apoptosis induced by serum deprivation in PC12 cells mediated by mitochondrial signaling pathway.
Application : WB
Reactivity : Rat
Wu DD et al. Oncotarget 2017; 8 (30):49144-49155 extracellular signal-regulated kinase 8-mediated nf-κb activation increases sensitivity of human lung cancer cells to *.
Omer FAA et al. Tumour Biol 2017; 39 (11):1.01E+15 Beta-mangostin from Cratoxylum arborescens activates the intrinsic apoptosis pathway through reactive oxygen species with downregulation of the HSP70 gene in the HL60 cells associated with a G0/G1 cell-cycle arrest.
Application : WB
Reactivity : Human
Lee WY et al. Int J Cancer 2017; 141 (9):1921-1931 * sensitizes kras-mutant non-small-cell lung cancer to * by targeting taz.
Application : WB
Reactivity : Human
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Application : WB
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Kuo HF et al. PLoS One 2016; 11 (5):e0156059 Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.
Application : WB
Reactivity : Human
Pezzani R et al. Invest New Drugs 2016; (Epub) The aurora kinase inhibitor VX-680 shows anti-cancer effects in primary metastatic cells and the SW13 cell line.
Application : WB
Reactivity : Human
Hsu WH et al. PLoS One 2015; 10 (4):e0121298 Evaluation of the medicinal herb Graptopetalum paraguayense as a treatment for liver cancer.
Application : WB
Reactivity : Human
Lin YC et al. Mediators Inflamm 2015; 2015:392471 Secretion of one adipokine Nampt/Visfatin suppresses the inflammatory stress-induced NF-κB activity and affects Nampt-dependent cell viability in Huh-7 cells.
Application : IP
Reactivity : Human
Chen PH et al. J Neurochem 2014; MicroRNA-302b-inhibited E2F3 transcription factor is related to all trans retinoic acid-induced glioma cell apoptosis.
Application : WB
Reactivity : Human
Chen JY et al. J Biomed Sci 2013; 20:95 quercetin-induced cardioprotection against * cytotoxicity.
Application : WB
Reactivity : Rat
Chen YW et al. Evid Based Complement Alternat Med 2013; 2013:364519 Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury.
Application : WB
Reactivity : Rat
Chen JH et al. Food Chem Toxicol 2013; 51:446-52 Acrylamide-induced mitochondria collapse and apoptosis in human astrocytoma cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169