GeneTex
United States (US)

Caspase 9 antibody

GTX31643_236_ICC-IF_20180221_18121410_713.jpg
GTX31643_2823_WB_20180221_18121410_922.jpg
GTX31643_237_ICC-IF_20180221_18121410_137.jpg
GTX31643_238_ICC-IF_20180221_18121410_617.jpg
GTX31643_235_ICC-IF_20180221_18121410_959.jpg

Cat No. GTX31643

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 5 μg/mL
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , HeLa Cell Lysate

Specificity/Sensitivity

Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Caspase-9 antibody was raised against a 20 amino acid peptide near the center of human Caspase-9.The immunogen is located within amino acids 290 - 340 of Caspase-9.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 9,Apaf-3,Apaf3,Ice-Lap6,Mch6,Ppp1R56,Casp9

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES
GTX31643_236_ICC-IF_20180221_18121410_713.jpg

GTX31643 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31643 Caspase 9 antibody.
Working concentration : 5 μg/ml

GTX31643_2823_WB_20180221_18121410_922.jpg

GTX31643 WB Image

WB analysis of HeLa cell lysate using GTX31643 Caspase 9 antibody.
Working concentration : 1 μg/ml

GTX31643_237_ICC-IF_20180221_18121410_137.jpg

GTX31643 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31643 Caspase 9 antibody.
Working concentration : 2 μg/ml

GTX31643_238_ICC-IF_20180221_18121410_617.jpg

GTX31643 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31643 Caspase 9 antibody.
Working concentration : 20 μg/ml

GTX31643_235_ICC-IF_20180221_18121410_959.jpg

GTX31643 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31643 Caspase 9 antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329