APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
K-562 Cell Slide(GTX25535) , HeLa Cell Lysate
Product Note
Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Caspase-9 antibody was raised against a 20 amino acid peptide near the center of human Caspase-9.The immunogen is located within amino acids 290 - 340 of Caspase-9.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caspase 9 , APAF-3 , APAF3 , ICE-LAP6 , MCH6 , PPP1R56
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Database
Research Area
DATA IMAGES
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GTX31643 WB Image
WB analysis of HeLa cell lysate using GTX31643 Caspase 9 antibody.
Working concentration : 1 μg/ml
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GTX31643 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31643 Caspase 9 antibody.
Working concentration : 5 μg/ml
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GTX31643 ICC/IF Image
ICC/IF analysis of K562 cells using GTX31643 Caspase 9 antibody.
Working concentration : 2 μg/ml
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GTX31643 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31643 Caspase 9 antibody.
Working concentration : 5 μg/ml
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GTX31643 ICC/IF Image
ICC/IF analysis of K562 cells using GTX31643 Caspase 9 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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