GeneTex
  • Country / Location Selection

United States (US)

Caspase 9 (cleaved Asp330) antibody

Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX132331

Cat No. GTX132331

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Jurkat (10 μM Etoposide treatment for 24 hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 9 (cleaved Asp330). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

caspase 9,APAF-3,APAF3,ICE-LAP6,MCH6,PPP1R56

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES
Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX132331

GTX132331 WB Image

Untreated (–) and treated (+) Jurkat whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 (cleaved Asp330) antibody (GTX132331) diluted at 1:500.

REFERENCE
REVIEW
There are currently no reviews for Caspase 9 (cleaved Asp330) antibody (GTX132331). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169