GeneTex
United States (US)

Caspase 9 (cleaved Asp330) antibody

Caspase-9-cleaved-Asp330-antibody-GTX86928-WB-1_18121409_643.jpg
Caspase-9-cleaved-Asp330-antibody-GTX86928-IHC-1_18121409_964.jpg

Cat No. GTX86928

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

17 and 37 kDa. ( Note )

Specificity/Sensitivity

Caspase 9 (Cleaved-Asp330) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The amino acid range of this immunogen is aa300-330 of human Caspase 9 with accession number P55211

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 9,Apaf-3,Apaf3,Ice-Lap6,Mch6,Ppp1R56,Casp9

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Database

Research Area

DATA IMAGES
Caspase-9-cleaved-Asp330-antibody-GTX86928-WB-1_18121409_643.jpg

GTX86928 WB Image

Western blot analysis of extracts from HeLa cells, treated with Etoposide 25uM 60', using Caspase 9 (Cleaved-Asp330) Antibody. The lane on the right is treated with the synthesized peptide.

Caspase-9-cleaved-Asp330-antibody-GTX86928-IHC-1_18121409_964.jpg

GTX86928 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Caspase 9 (Cleaved-Asp330) Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339