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Caspase 9 (cleaved Asp330) antibody

Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX86928
Anti-Caspase 9 (cleaved Asp330) antibody used in IHC (Paraffin sections) (IHC-P). GTX86928

Cat No. GTX86928

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μg ($339)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Specificity/Sensitivity

Caspase 9 (cleaved Asp330) antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 9 (281-330).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APAF3,CASP9,ICELAP6,MCH6,PPP1R56,caspase 9,Caspase 9

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES
Anti-Caspase 9 (cleaved Asp330) antibody used in Western Blot (WB). GTX86928

GTX86928 WB Image

WB analysis of HeLa cells treated with Etoposide 25uM (60mins) lysate using GTX86928 Caspase 9 (cleaved Asp330) antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Caspase 9 (cleaved Asp330) antibody used in IHC (Paraffin sections) (IHC-P). GTX86928

GTX86928 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86928 Caspase 9 (cleaved Asp330) antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE
There are currently no references for Caspase 9 (cleaved Asp330) antibody (GTX86928). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 339