APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500~1:1000 |
| 1:50~1:100 |
Not tested in other applications.
Calculated MW
Product Note
Caspase 9 (cleaved Asp330) antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 9 (281-330).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
APAF3 , CASP9 , ICELAP6 , MCH6 , PPP1R56 , caspase 9 , Caspase 9
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Database
Research Area
DATA IMAGES
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GTX86928 WB Image
WB analysis of HeLa cells treated with Etoposide 25uM (60mins) lysate using GTX86928 Caspase 9 (cleaved Asp330) antibody. The lane on the right is blocked with the synthesized peptide.
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GTX86928 IHC-P Image
IHC-P analysis of human lung carcinoma tissue using GTX86928 Caspase 9 (cleaved Asp330) antibody. The picture on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for Caspase 9 (cleaved Asp330) antibody (GTX86928). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Caspase 9 (cleaved Asp330) antibody (GTX86928). Be the first to share your experience with this product.
