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Caspase 9 (cleaved Asp353) antibody

Orthogonal
Anti-Caspase 9 (cleaved Asp353) antibody used in Western Blot (WB). GTX86912
Anti-Caspase 9 (cleaved Asp353) antibody used in IHC (Paraffin sections) (IHC-P). GTX86912
Anti-Caspase 9 (cleaved Asp353) antibody used in Western Blot (WB). GTX86912

Cat. No. GTX86912

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all Caspase 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Product Note

Caspase 9 (cleaved Asp353) antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp353.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from mouse Caspase 9 (323-372).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10724147

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

plays a role in initiation and progression of apoptosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Caspase 9 (cleaved Asp353) antibody used in Western Blot (WB). GTX86912

GTX86912 WB Image

WB analysis of HEPG2-UV cell lysates using GTX86912 Caspase 9 (cleaved Asp353) antibody.
Dilution : 1:1000

Anti-Caspase 9 (cleaved Asp353) antibody used in IHC (Paraffin sections) (IHC-P). GTX86912

GTX86912 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX86912 Caspase 9 (cleaved Asp353) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Caspase 9 (cleaved Asp353) antibody used in Western Blot (WB). GTX86912

GTX86912 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2016. PMID: 27251104

REFERENCE

Application Reference

Ji X et al. FASEB J 2020; 34 (4) : 4904-4917 WISP-2, an Upregulated Gene in Hip Cartilage From the DDH Model Rats, Induces Chondrocyte Apoptosis Through PPARγ in Vitro.
Application : WB
Reactivity : Rat
Kutikhin AG et al. Sci Rep 2016; 6 : 27255 Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399