Western blot using GeneTex's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 μg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa).

Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20μg). Lane 2: NBLS Nuclear (3μg). Lane 3: BE2C Cytoplasmic (30μg). Lane 4: BE2C Nuclear (7μg). Lane 5: SY5Y-CASZ1b (10μg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.