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Casz1 antibody

Anti-Casz1 antibody used in Western Blot (WB). GTX48704
Anti-Casz1 antibody used in Western Blot (WB). GTX48704

Cat No. GTX48704

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Drosophila, Chimpanzee, Macaque
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:5000
ELISA 1:45000

Note :

WB
Detects both the hCASZ5 and hCASZ11 isoforms. Expect a band ~ 125 kDa for hCASZ5 and 190 kDa for hCASZ11.

Not tested in other applications.

Calculated MW

190 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

castor zinc finger 1 , CAS11 , CST , SRG , ZNF693 , dJ734G22.1

Cellular Localization

Nucleus

Background

The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.

Database

Research Area

DATA IMAGES

Anti-Casz1 antibody used in Western Blot (WB). GTX48704

GTX48704 WB Image

Western blot using GeneTex's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 μg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa).

Anti-Casz1 antibody used in Western Blot (WB). GTX48704

GTX48704 WB Image

Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20μg). Lane 2: NBLS Nuclear (3μg). Lane 3: BE2C Cytoplasmic (30μg). Lane 4: BE2C Nuclear (7μg). Lane 5: SY5Y-CASZ1b (10μg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf