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Casz1 antibody

Anti-Casz1 antibody used in Western Blot (WB). GTX48704
Anti-Casz1 antibody used in Western Blot (WB). GTX48704

Cat. No. GTX48704

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, ChIP assay, Multiplexing

Reactivity

 Human
Package
50 μg ($399)
See all CASZ1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA 1:45000
ChIP assay Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

190 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region of Human Casz1 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

castor zinc finger 1 , CAS11 , CST , SRG , ZNF693 , dJ734G22.1

Cellular Localization

Nucleus

Background

The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.

Database

Research Area

DATA IMAGES

Anti-Casz1 antibody used in Western Blot (WB). GTX48704

GTX48704 WB Image

Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20μg). Lane 2: NBLS Nuclear (3μg). Lane 3: BE2C Cytoplasmic (30μg). Lane 4: BE2C Nuclear (7μg). Lane 5: SY5Y-CASZ1b (10μg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.

Anti-Casz1 antibody used in Western Blot (WB). GTX48704

GTX48704 WB Image

Western blot using GeneTex's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 μg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa).

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399