GeneTex
United States (US)

Catalase antibody

GTX64374_20180530_IP_18121411_841.jpg
GTX64374_20180530_WB_18121411_359.jpg

Cat No. GTX64374

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:3000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-225 of human CAT (NP_001743.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CAT Antibody

Background

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX64374_20180530_IP_18121411_841.jpg

GTX64374 IP Image

IP analysis of HepG2 cell lysate using GTX64374 Catalase antibody.
IP reaction : 3μg antibody / 100μg cell lysate
Dilution : 1:1000

GTX64374_20180530_WB_18121411_359.jpg

GTX64374 WB Image

WB analysis of various samples using GTX64374 Catalase antibody.
Dilution : 1:3000
Loading : 25μg per lane