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Cathepsin A antibody

Anti-Cathepsin A antibody used in Western Blot (WB). GTX133016
Anti-Cathepsin A antibody used in Western Blot (WB). GTX133016
Anti-Cathepsin A antibody used in IHC (Paraffin sections) (IHC-P). GTX133016

Cat No. GTX133016

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

U87-MG , DDDDK-tagged CTSA-transfected 293T

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cathepsin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cathepsin A , GLB2 , GSL , NGBE , PPCA , PPGB

Cellular Localization

Lysosome

Background

This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]

Database

DATA IMAGES

Anti-Cathepsin A antibody used in Western Blot (WB). GTX133016

GTX133016 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin A antibody (GTX133016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cathepsin A antibody used in Western Blot (WB). GTX133016

GTX133016 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin A antibody (GTX133016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cathepsin A antibody used in IHC (Paraffin sections) (IHC-P). GTX133016

GTX133016 IHC-P Image

Cathepsin A antibody detects Cathepsin A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Cathepsin A stained by Cathepsin A antibody (GTX133016) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169