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Cathepsin B antibody

Comparison Orthogonal Overexpression
Anti-Cathepsin B antibody used in Western Blot (WB). GTX134724
Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX134724
Anti-Cathepsin B antibody used in Western Blot (WB). GTX134724

Cat. No. GTX134724

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cathepsin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Cathepsin B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887332

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cathepsin B , APPS , CPSB , RECEUP

Cellular Localization

Lysosome

Background

This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-Cathepsin B antibody used in Western Blot (WB). GTX134724

GTX134724 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin B antibody (GTX134724) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX134724

GTX134724 IHC-P Image

Cathepsin B antibody detects Cathepsin B protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
Cathepsin B stained by Cathepsin B antibody (GTX134724) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cathepsin B antibody used in Western Blot (WB). GTX134724

GTX134724 WB Image

Comparison Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Cathepsin B antibody (GTX134724) diluted at 1:1000 and competitor's antibody (CST#31718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169