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Cathepsin B antibody

Anti-Cathepsin B antibody used in Western Blot (WB). GTX64404
Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX64404
Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX64404
Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX64404

Cat. No. GTX64404

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cathepsin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human CTSB (NP_001899.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888598

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cathepsin B , APPS , CPSB , RECEUP

Cellular Localization

Lysosome

Background

This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-Cathepsin B antibody used in Western Blot (WB). GTX64404

GTX64404 WB Image

WB analysis of various sample lysates using GTX64404 Cathepsin B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX64404

GTX64404 IHC-P Image

IHC-P analysis of mouse spinal cord tissue using GTX64404 Cathepsin B antibody.
Dilution : 1:50

Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX64404

GTX64404 IHC-P Image

IHC-P analysis of human liver tissue using GTX64404 Cathepsin B antibody.
Dilution : 1:50

Anti-Cathepsin B antibody used in IHC (Paraffin sections) (IHC-P). GTX64404

GTX64404 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64404 Cathepsin B antibody.
Dilution : 1:50

REFERENCE

Application Reference

Ho KH et al. Pharmacol Res 2019; 147 : 104390 miR-140 targeting CTSB signaling suppresses the mesenchymal transition and enhances temozolomide cytotoxicity in glioblastoma multiforme.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399