GeneTex
United States (US)

Cathepsin C (cleaved Arg394) antibody

Dipeptidyl-peptidase-1-cleaved-Arg394-antibody-GTX86919-WB-1_18121410_734.jpg

Cat No. GTX86919

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

27 kDa. ( Note )

Specificity/Sensitivity

cathepsin C (heavy chain,Cleaved-Arg394) Antibody detects endogenous levels of fragment of activated cathepsin C resulting from cleavage adjacent to Arg394.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human cathepsin C.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cathepsin C,Cppi,Dpp-I,Dpp1,Dppi,Hms,Jp,Jpd,Pals,Pdon1,Pls,Ctsc

Background

Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B. Can also activate neuraminidase and factor XIII.

Database

Research Area

DATA IMAGES
Dipeptidyl-peptidase-1-cleaved-Arg394-antibody-GTX86919-WB-1_18121410_734.jpg

GTX86919 WB Image

Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 1h, using Dipeptidyl-peptidase 1 (heavy chain,Cleaved-Arg394) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339