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Cathepsin D antibody [4G2]

Anti-Cathepsin D antibody [4G2] used in IHC (Paraffin sections) (IHC-P). GTX53801
Anti-Cathepsin D antibody [4G2] used in Western Blot (WB). GTX53801

Cat No. GTX53801

Host

Mouse

Clonality

Monoclonal

Clone Name

4G2

Isotype

IgG2b

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Cathepsin D (21-412 aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cathepsin D , Cln10 , Cpsd , Hel-S-130P , Ctsd

Background

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cathepsin D antibody [4G2] used in IHC (Paraffin sections) (IHC-P). GTX53801

GTX53801 IHC-P Image

IHC-P analysis of human breast lobule tissue using Cathepsin D antibody at a dilution of 1:50.

Anti-Cathepsin D antibody [4G2] used in Western Blot (WB). GTX53801

GTX53801 WB Image

WB analysis of HepG2, Hep3B, MDA-MB-231 and MCF7 lysates (20ug per lane) using Cathepsin D antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf