GeneTex
United States (US)

Cathepsin D (cleaved Leu169) antibody

CATD-cleaved-Leu169-antibody-GTX86942-WB-1_18121409_929.jpg

Cat No. GTX86942

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:40000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

27 kDa. ( Note )

Specificity/Sensitivity

CATD (heavy chain,Cleaved-Leu169) Antibody detects endogenous levels of fragment of activated CATD resulting from cleavage adjacent to Leu169.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CATD.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cathepsin D,Cln10,Cpsd,Hel-S-130P,Ctsd

Cellular Localization

Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Background

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Database

Research Area

DATA IMAGES
CATD-cleaved-Leu169-antibody-GTX86942-WB-1_18121409_929.jpg

GTX86942 WB Image

Western blot analysis of extracts from 293/A549/JurKat cells, treated with etoposide 25uM 1h, using CATD (heavy chain,Cleaved-Leu169) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339