GeneTex
United States (US)

Cathepsin G (cleaved Ile21) antibody

CATG-cleaved-Ile21-antibody-GTX86950-WB-1_18121409_233.jpg

Cat No. GTX86950

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

29 and 36 kDa. ( Note )

Specificity/Sensitivity

CATG (Cleaved-Ile21) Antibody detects endogenous levels of fragment of activated CATG resulting from cleavage adjacent to Ile21.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CATG.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cathepsin G,Catg,Cg,Ctsg

Background

Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.

Database

Research Area

DATA IMAGES
CATG-cleaved-Ile21-antibody-GTX86950-WB-1_18121409_233.jpg

GTX86950 WB Image

Western blot analysis of extracts from HepG2/COLO/HuvEc/A549 cells, using CATG (Cleaved-Ile21) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339