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Cathepsin L antibody [33/2]

Cat. No. GTX26314

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 33/2

Isotype

 IgG1

Application

 WB, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Mink
Package
100 μl ($399)
See all Cathepsin L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

mink lung cell

Product Note

Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_374384

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cathepsin L , CATL , CTSL1 , MEP

Cellular Localization

Lysosome

Background

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intra-lysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive pro-form, and mRNA expression level seems to correlate with their metastatic potential.

Database

Research Area

REFERENCE

Application Reference

Sa?kowska A et al. Cells 2020; 9 (7) Identification of Novel Molecular Markers of Human Th17 Cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399