APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500~1:1000 |
Not tested in other applications.
Calculated MW
Product Note
Cathepsin L (cleaved Thr288) antibody detects endogenous levels of fragment of activated CATL1 resulting from cleavage adjacent to Thr288.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human Cathepsin L (239-288).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CATL , CTSL , CTSL1 , MEP , cathepsin L , Cathepsin L
Cellular Localization
Lysosome
Background
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
|
GTX86946 WB Image
WB analysis of HeLa cells treated with etoposide 25uM (1h) lysate using GTX86946 Cathepsin L (cleaved Thr288) antibody. The lane on the right is blocked with the synthesized peptide.
|
REFERENCE
There are currently no references for Cathepsin L (cleaved Thr288) antibody (GTX86946). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cathepsin L (cleaved Thr288) antibody (GTX86946). Be the first to share your experience with this product.