GeneTex
United States (US)

Cathepsin O antibody [N1C3]

GTX105671_WB_19011506_408.jpg
GTX105671_39897_IFA_19011506_658.jpg
GTX105671_39897_IHC_19011506_263.jpg

Cat No. GTX105671

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2 , HCT116
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cathepsin O. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTSO1 antibody, CTSO antibody, cathepsin O antibody

Background

The protein encoded by the gene is a cysteine proteinase and a member of the papain superfamily. This proteolytic enzyme is involved in cellular protein degradation and turnover. The recombinant form of this enzyme was shown to degrade synthetic peptides typically used as substrates for cysteine proteinases and its proteolytic activity was abolished by an inhibitor of cyteine proteinase. [provided by RefSeq]
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Database

DATA IMAGES
GTX105671_WB_19011506_408.jpg

GTX105671 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX105671 diluted at 1:1000

GTX105671_39897_IFA_19011506_658.jpg

GTX105671 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using Cathepsin O(GTX105671) antibody at 1:500 dilution.

GTX105671_39897_IHC_19011506_263.jpg

GTX105671 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Cathepsin O(GTX105671) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169