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Cathepsin S antibody [HL2302]

Anti-Cathepsin S antibody [HL2302] used in IHC (Paraffin sections) (IHC-P). GTX638369
Anti-Cathepsin S antibody [HL2302] used in Western Blot (WB). GTX638369
Anti-Cathepsin S antibody [HL2302] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638369
Anti-Cathepsin S antibody [HL2302] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638369
Anti-Cathepsin S antibody [HL2302] used in IHC (Paraffin sections) (IHC-P). GTX638369

Cat. No. GTX638369

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2302

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Cathepsin S.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cathepsin S

Cellular Localization

Lysosome

Background

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-Cathepsin S antibody [HL2302] used in IHC (Paraffin sections) (IHC-P). GTX638369

GTX638369 IHC-P Image

Cathepsin S antibody [HL2302] detects Cathepsin S protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tissue.
Cathepsin S stained by Cathepsin S antibody [HL2302] (GTX638369) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cathepsin S antibody [HL2302] used in Western Blot (WB). GTX638369

GTX638369 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cathepsin S antibody [HL2302] (GTX638369) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Cathepsin S antibody [HL2302] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638369

GTX638369 ICC/IF Image

Cathepsin S antibody [HL2302] detects Cathepsin S protein at fiber by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cathepsin S stained by Cathepsin S antibody [HL2302] (GTX638369) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Cathepsin S antibody [HL2302] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638369

GTX638369 ICC/IF Image

Cathepsin S antibody [HL2302] detects Cathepsin S protein at cytoplasmic vesicle by immunofluorescent analysis.
Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.
Green: Cathepsin S stained by Cathepsin S antibody [HL2302] (GTX638369) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Cathepsin S antibody [HL2302] used in IHC (Paraffin sections) (IHC-P). GTX638369

GTX638369 IHC-P Image

Cathepsin S antibody [HL2302] detects Cathepsin S protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Cathepsin S stained by Cathepsin S antibody [HL2302] (GTX638369) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169