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Cathepsin S antibody

Anti-Cathepsin S antibody used in IHC (Paraffin sections) (IHC-P). GTX103988
Anti-Cathepsin S antibody used in Western Blot (WB). GTX103988
Anti-Cathepsin S antibody used in Western Blot (WB). GTX103988

Cat No. GTX103988

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa , rat brain

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cathepsin S. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cathepsin S

Cellular Localization

Lysosome

Background

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Cathepsin S antibody used in IHC (Paraffin sections) (IHC-P). GTX103988

GTX103988 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using CTSS(GTX103988) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cathepsin S antibody used in Western Blot (WB). GTX103988

GTX103988 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin S antibody (GTX103988) diluted at 1:1000.

Anti-Cathepsin S antibody used in Western Blot (WB). GTX103988

GTX103988 WB Image

Cathepsin S antibody detects CTSS protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
10 % SDS-PAGE
Cathepsin S antibody (GTX103988) dilution: 1:1000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf