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Cav beta 1 antibody [C1C3]

Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807
Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807
Anti-Cav beta 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108807
Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807
Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807

Cat. No. GTX108807

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cav beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , HepG2 , Raji , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cav beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036401

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium voltage-gated channel auxiliary subunit beta 1 , CAB1 , CACNLB1 , CCHLB1

Cellular Localization

Cell membrane , sarcolemma

Background

The protein encoded by this gene belongs to the calcium channel beta subunit family. It plays an important role in the calcium channel by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807

GTX108807 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cav beta 1 antibody (GTX108807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807

GTX108807 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Hep G2 (GTX27900)
C: Raji
7.5% SDS PAGE
GTX108807 diluted at 1:1000

Anti-Cav beta 1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108807

GTX108807 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CACNB1(GTX108807) antibody at 1:200 dilution.

Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807

GTX108807 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cav beta 1 antibody [C1C3] (GTX108807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cav beta 1 antibody [C1C3] used in Western Blot (WB). GTX108807

GTX108807 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108807 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169