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Cav beta 2 antibody

Anti-Cav beta 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131906
Anti-Cav beta 2 antibody used in Western Blot (WB). GTX131906

Cat No. GTX131906

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rabbit, Bovine, Guinea pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Cav beta 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calcium voltage-gated channel auxiliary subunit beta 2,CACNLB2,CAVB2,MYSB

Background

This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Feb 2013]

Database

Research Area

DATA IMAGES
Anti-Cav beta 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131906

GTX131906 IHC-Fr Image

Cav beta 2 antibody detects Cav beta 2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: Cav beta 2 stained by Cav beta 2 antibody (GTX131906) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-Cav beta 2 antibody used in Western Blot (WB). GTX131906

GTX131906 WB Image

Various whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cav beta 2 antibody (GTX131906) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169