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Cav1.2 antibody

Anti-Cav1.2 antibody used in Western Blot (WB). GTX54754
Anti-Cav1.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54754
Anti-Cav1.2 antibody used in Western Blot (WB). GTX54754

Cat No. GTX54754

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS, IP

Reactivity

Human, Mouse, Rat
Package
50 μl ($549)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

243 kDa. ( Note )

Predict Reactivity

Rabbit, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 (Intracellular loop between domains II and III) of rat CaV1.2 (Accession  : P22002).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium voltage-gated channel subunit alpha1 C , RATIVS302

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

subunit of an L-type calcium channel that may be involved in bone metabolism and compensatory renal growth [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Cav1.2 antibody used in Western Blot (WB). GTX54754

GTX54754 WB Image

WB analysis of rat brain membrane lysate using GTX54754 Cav1.2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Cav1.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54754

GTX54754 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX54754 Cav1.2 antibody.
Panel A : CaV1.2 (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows).
Panel B : Staining of Purkinje nerve cells with mouse anti-Calbindin 28K  (green) demonstrates the location of dendrites in the molecular layer.
Panel C : Merged image of panels A and B.

Anti-Cav1.2 antibody used in Western Blot (WB). GTX54754

GTX54754 WB Image

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf