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Cav2.1 antibody

Anti-Cav2.1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54753
Anti-Cav2.1 antibody used in Western Blot (WB). GTX54753

Cat No. GTX54753

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat
Package
50 μl ($549)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

252 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 (Intracellular loop between domains II and III) of rat CACNA1A (Accession  : P54282).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium voltage-gated channel subunit alpha1 A , BccA1 , Cav2.1 , rbA-1

Cellular Localization

Cell membrane

Background

voltage-gated calcium channel that may be involved in secretion coupling in pancreatic beta cells [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Cav2.1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54753

GTX54753 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX54753 Cav2.1 antibody.
Panel A : CACNA1A  channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows).
Panel B : Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer.
Panel C : Merged image of panels A and B.
Dilution : 1:100

Anti-Cav2.1 antibody used in Western Blot (WB). GTX54753

GTX54753 WB Image

WB analysis of rat brain membrane lysate using GTX54753 Cav2.1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf