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Cav2.2 antibody [C3], C-term

Anti-Cav2.2 antibody [C3], C-term used in Western Blot (WB). GTX102449
Anti-Cav2.2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102449

Cat No. GTX102449

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

262 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Human Cav2.2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium voltage-gated channel subunit alpha1 B , BIII , CACNL1A5 , CACNN , Cav2.2 , DYT23

Cellular Localization

Membrane

Background

Voltage-dependent Ca(2+) channels are multisubunit complexes found in the membrane of many excitable cells that regulate calcium entry (see MIM 601011). N-type calcium channels, which control neurotransmitter release from neurons, are dihydropyridine-insensitive and omega-conotoxin-sensitive. The alpha-1 subunit forms the pore through which calcium enters the cell, and is encoded by a family of at least 5 genes.[supplied by OMIM]

Database

  • Gene ID: 774 CACNA1B
  • UniProt: Q00975 CACNA1B

Research Area

DATA IMAGES

Anti-Cav2.2 antibody [C3], C-term used in Western Blot (WB). GTX102449

GTX102449 WB Image

CACNA1B antibody [C3], C-term detects CACNA1B protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
5% SDS-PAGE
CACNA1B antibody [C3], C-term (GTX102449) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cav2.2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102449

GTX102449 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using CACNA1B(GTX102449) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169