APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.37 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human Cav2.2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240530
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium voltage-gated channel subunit alpha1 B , BIII , CACNL1A5 , CACNN , Cav2.2 , DYT23
Cellular Localization
Membrane; Multi-pass membrane protein
Background
The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
|
GTX102449 ICC/IF Image
Cav2.2 antibody [C3], C-term detects Cav2.2 protein at cell membrane and nucleus by immunofluorescent analysis.Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cav2.2 stained by Cav2.2 antibody [C3], C-term (GTX102449) diluted at 1:500.Red: NF-H, a axon marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
|
GTX102449 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Cav2.2 antibody [C3], C-term (GTX102449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Cav2.2 antibody [C3], C-term (GTX102449). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cav2.2 antibody [C3], C-term (GTX102449). Be the first to share your experience with this product.