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Cav2.2 antibody

Anti-Cav2.2 antibody used in Western Blot (WB). GTX54812
Anti-Cav2.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54812
Anti-Cav2.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54812

Cat. No. GTX54812

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Cav2.2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 ( Intracellular loop between domains II and III) of rat CACNA1B (Accession  : Q02294).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium voltage-gated channel subunit alpha1 B , BIII

Database

Research Area

DATA IMAGES

Anti-Cav2.2 antibody used in Western Blot (WB). GTX54812

GTX54812 WB Image

WB analysis of rat brain membrane lysate using GTX54812 Cav2.2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Cav2.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54812

GTX54812 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX54812 Cav2.2 antibody.
Panel A : CaV2.2 (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol).
Panel B : Staining of Purkinje cells with mouse anti-Calbindin 28K (green) demonstrates the restriction of CaV2.2 to cell bodies but not to dendrites in the molecular layer.
Panel C : Merged image of panels A and B.
Dilution : 1:100

Anti-Cav2.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54812

GTX54812 ICC/IF Image

ICC/IF analysis of PFA-fixed rat DRG primary cells using GTX54812 Cav2.2 antibody.
Panel A : Primary antibody
Panel B : DNA dye Hoechst 33342
Panel C : Merged images of panels A and B
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639