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Cav3.2 antibody

Anti-Cav3.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54813
Anti-Cav3.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54813
Anti-Cav3.2 antibody used in Western Blot (WB). GTX54813
Anti-Cav3.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54813

Cat No. GTX54813

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
50 μl ($549)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

261 kDa. ( Note )

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 (Intracellular loop between domains D1 and D2) of rat CaV3.2 (Accession number Q9EQ60).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium voltage-gated channel subunit alpha1 H

Cellular Localization

Cell membrane

Background

acts as a low voltage gated calcium channel; conducts calcium current; involved in neuronal physiology [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Cav3.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54813

GTX54813 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54813 Cav3.2 antibody. Staining is specific for DRG. Note that neither glial cells nor axonal fibers are stained. Hoechst 33342 is used as the counterstain.
Dilution : 1:50

Anti-Cav3.2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54813

GTX54813 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX54813 Cav3.2 antibody.
Panel A : CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red).
Panel B : Staining of Purkinje cells with mouse anti-parvalbumin (PV, green).
Panel C : Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinje  cells.
Dilution : 1:100

Anti-Cav3.2 antibody used in Western Blot (WB). GTX54813

GTX54813 WB Image

WB analysis of rat DRG lysate using GTX54813 Cav3.2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Cav3.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54813

GTX54813 ICC/IF Image

ICC/IF analysis of PFA-fixed rat DRG primary cells using GTX54813 Cav3.2 antibody.
Panel A,D : Primary antibody
Panel B,E : DNA dye Hoechst 33342
Panel C,F : Merged images of panels A and B
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf