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Caveolin 1 antibody [7C8]

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350
Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Western Blot (WB). GTX79350
Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

Cat. No. GTX79350

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7C8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat, Hamster
Package
100 μl ($399)
See all Caveolin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1:125
IHC-P 1:20
FACS Assay dependent
IP 1 - 3 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Intercellular membrane protein-containing vesicles (containing GLUT4) from rat.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11175607

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caveolin 1 , Cav

Background

structural protein of lipid raft domains in the membrane called caveolae; functions as a cholesterol-binding/shuttling protein [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

GTX79350 FACS Image

FACS analysis of CHO cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

GTX79350 ICC/IF Image

ICC/IF analysis of H-4-II-E Cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

GTX79350 FACS Image

FACS analysis of H-4-II-E cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350

GTX79350 IHC-P Image

IHC-P analysis of rat lung tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Caveolin 1 antibody [7C8] used in Western Blot (WB). GTX79350

GTX79350 WB Image

WB analysis of 25 ug of A431 (Lane 1) and rat lung (Lane 2) cell lysates using GTX79350 Caveolin 1 antibody [7C8].
Dilution : 1:1000

Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350

GTX79350 IHC-P Image

IHC-P analysis of rat lymph node tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

GTX79350 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for 1 hour at room temperature

Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

GTX79350 ICC/IF Image

ICC/IF analysis of cells using GTX79350 Caveolin 1 antibody [7C8].

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

GTX79350 FACS Image

FACS analysis of L6 cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

Application Reference

Liu IF et al. Biol Direct 2023; 18 (1) : 9 Long-term administration of Western diet induced metabolic syndrome in mice and causes cardiac microvascular dysfunction, cardiomyocyte mitochondrial damage, and cardiac remodeling involving caveolae and caveolin-1 expression.
Application : WB, IHC-P
Reactivity : Mouse
Ise H et al. Genes Cells 2017; (Epub) Elucidation of GlcNAc-binding properties of type III intermediate filament proteins, using GlcNAc-bearing polymers.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399