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Caveolin 1 antibody [7C8]

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Western Blot (WB). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350
Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350
Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350
Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

Cat No. GTX79350

Host

Mouse

Clonality

Monoclonal

Clone Name

7C8

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human, Rat, Hamster
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:2000
ICC/IF 1:125
IHC-P 1:20
FACS Assay dependent
IP 1 - 3 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Intercellular membrane protein-containing vesicles (containing GLUT4) from rat.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11175607

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caveolin 1 , Cav

Background

structural protein of lipid raft domains in the membrane called caveolae; functions as a cholesterol-binding/shuttling protein [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

GTX79350 FACS Image

FACS analysis of CHO cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

GTX79350 ICC/IF Image

ICC/IF analysis of cells using GTX79350 Caveolin 1 antibody [7C8].

Anti-Caveolin 1 antibody [7C8] used in Western Blot (WB). GTX79350

GTX79350 WB Image

WB analysis of 25 ug of A431 (Lane 1) and rat lung (Lane 2) cell lysates using GTX79350 Caveolin 1 antibody [7C8].
Dilution : 1:1000

Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

GTX79350 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for 1 hour at room temperature

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

GTX79350 FACS Image

FACS analysis of H-4-II-E cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350

GTX79350 IHC-P Image

IHC-P analysis of rat lymph node tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Caveolin 1 antibody [7C8] used in IHC (Paraffin sections) (IHC-P). GTX79350

GTX79350 IHC-P Image

IHC-P analysis of rat lung tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Caveolin 1 antibody [7C8] used in Flow cytometry (FACS). GTX79350

GTX79350 FACS Image

FACS analysis of L6 cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Caveolin 1 antibody [7C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79350

GTX79350 ICC/IF Image

ICC/IF analysis of H-4-II-E Cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf