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Caveolin 1 antibody, Internal

Anti-Caveolin 1 antibody, Internal used in Western Blot (WB). GTX89541
Anti-Caveolin 1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89541

Cat. No. GTX89541

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Hamster
Package
100 μg ($399)
See all Caveolin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89541-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_001744.2; NP_001166366.1).

Predict Reactivity

Mouse, Rat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2; NP_001166366.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723064

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caveolin 1 , BSCL3 , CGL3 , LCCNS , MSTP085 , PPH3 , VIP21

Background

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-Caveolin 1 antibody, Internal used in Western Blot (WB). GTX89541

GTX89541 WB Image

WB analysis of human spleen lysate using GTX89541 Caveolin 1 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Caveolin 1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89541

GTX89541 IHC-P Image

IHC-P analysis of human heart using GTX89541 Caveolin 1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

Application Reference

Rosales Ramirez R et al. J Virol 2019; 93 (4) The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex.
Romel Rosales Ramirez et al. bioRxiv 2018; (Epub) The dengue virus non-structural protein 1 (NS1) is secreted from mosquito cells in association with the intracellular cholesterol transporter chaperone caveolin complex.
Application : ICC/IF
Reactivity : Hamster
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399