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Caveolin 1 antibody [N1N3]

KO/KD
GTX100205_39274_20170525_WB_KO_watermark_19011506_532.jpg
GTX100205_IHC_19011506_110.jpg
GTX100205_39274_20141231_WB_19011506_607.jpg
GTX100205_39274_20151029_WB_M_lung_19011506_336.jpg
GTX100205_39274_20150106_IP_19011506_155.jpg
GTX100205_39274_IFA_19011506_806.jpg

Cat No. GTX100205

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , *A549 , *A7 , Mouse lung

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BSCL3 antibody, CGL3 antibody, MSTP085 antibody, VIP21 antibody, CAV1 antibody, cell growth-inhibiting protein 32 antibody, caveolin-1 antibody, "caveolin 1, caveolae protein, 22kDa antibody"

Cellular Localization

Golgi apparatus membrane , Cell membrane , Membrane , caveola

Background

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100205_39274_20170525_WB_KO_watermark_19011506_532.jpg

GTX100205 WB Image

KO/KD

Wild-type (WT) and Caveolin 1 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX100205_IHC_19011506_110.jpg

GTX100205 IHC-P Image

Immunohistochemical microscopy analysis of paraffin-embedded lung carcinoma cell line xenograft tissue using Caveolin-1 antibody [N1N3] (GTX100205) (1:100).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100205_39274_20141231_WB_19011506_607.jpg

GTX100205 WB Image

Caveolin 1 antibody detects Caveolin 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody (GTX100205) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100205_39274_20151029_WB_M_lung_19011506_336.jpg

GTX100205 WB Image

Caveolin 1 antibody [N1N3] detects Caveolin 1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100205_39274_20150106_IP_19011506_155.jpg

GTX100205 IP Image

Immunoprecipitation of Caveolin 1 protein from A549 membrane extracts using 5 μg of Caveolin 1 antibody [N1N3] (GTX100205).
Western blot analysis was performed using Caveolin 1 antibody [N1N3] (GTX100205).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100205_39274_IFA_19011506_806.jpg

GTX100205 ICC/IF Image

Caveolin 1 antibody [N1N3] detects Caveolin 1 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Caveolin 1 protein stained by Caveolin 1 antibody [N1N3] (GTX100205) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE
REVIEW

Caveolin 1 antibody [N1N3] Cat No. GTX100205

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 3 users reviews)
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells, T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: A549 cells, Lane 2: T24 cells.
Date : Anonymous submitted on 17-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : A7 cells, HeLa cells
Amount used : 15μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, overnightHr
Lane Description : Lane 1:A7 cells. Lane 2:HeLa cells.
Date : Anonymous submitted on 04-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 60μg
Blocking : 5% non-fat milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf