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Caveolin 1 (phospho Tyr14) antibody

Anti-Caveolin 1 (phospho Tyr14) antibody used in Western Blot (WB). GTX32313

Cat No. GTX32313

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Caveolin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caveolin 1,Bscl3,Cgl3,Lccns,Mstp085,Pph3,Vip21,Cav1

Cellular Localization

Caveola,Cell membrane,Golgi apparatus,Golgi apparatus membrane,Membrane,Membrane raft,Trans-Golgi network

Background

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES
Anti-Caveolin 1 (phospho Tyr14) antibody used in Western Blot (WB). GTX32313

GTX32313 WB Image

WB analysis of A549 (A), pervanadate-treated HeLa (B) whole cell lysates using GTX32313 Caveolin 1 (phospho Tyr14) antibody.