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Caveolin 2 (phospho Tyr19) antibody

Orthogonal
Anti-Caveolin 2 (phospho Tyr19) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23417
Anti-Caveolin 2 (phospho Tyr19) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23417

Cat. No. GTX23417

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Caveolin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1-2 μg/ml
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic phosphopeptide corresponding to residues M(14) A D D A (pY) S H H S G C(25) of mouse CAV2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caveolin 2 , AI447843

Cellular Localization

Nucleus,Cytoplasm

Background

This gene belongs to the caveolin family whose members encode the major protein components of caveolae, which are invaginations of plasma membrane. This gene is located adjacent to caveolin-1 and the proteins coexpressed by the two genes localize together in caveolae, where they form hetero-oligomers. The encoded protein may be involved in diverse cellular functions including proliferation, differentiation, endocytosis and trafficking. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]

Database

Research Area

DATA IMAGES

Anti-Caveolin 2 (phospho Tyr19) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23417

GTX23417 ICC/IF Image

Orthogonal

ICC/IF analysis of HUVEC cells treated with 100μM pervanadate (right) and untreated cells (left) using GTX23417 Caveolin 2 (phospho Tyr19) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-Caveolin 2 (phospho Tyr19) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23417

GTX23417 ICC/IF Image

ICC/IF analysis of A375 Cells using GTX23417 Caveolin 2 (phospho Tyr19) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399