ICC/IF analysis of A375 Cells using GTX23417 Caveolin 2 (phospho Tyr19) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

ICC/IF analysis of HUVEC cells treated with 100μM pervanadate (right) and untreated cells (left) using GTX23417 Caveolin 2 (phospho Tyr19) antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC