GeneTex
United States (US)

Caveolin 3 antibody, N-term

GTX88437_2328_WB_18121411_760.jpg
GTX88437_2327_WB_18121411_205.jpg
GTX88437_2329_WB_18121411_723.jpg
GTX88437_962_IHC-P_18121411_735.jpg

Cat No. GTX88437

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Skeletal Muscle shows peripheral staining of fibres.

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88437-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence EHTDLEAQIVKDIH-C, from the N Terminus of the protein sequence according to NP_203123.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caveolin 3,Lgmd1C,Lqt9,Vip-21,Vip21,Cav3

Cellular Localization

Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Background

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88437_2328_WB_18121411_760.jpg

GTX88437 WB Image

WB analysis of human heart lysate using GTX88437 Caveolin 3 antibody, N-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88437_2327_WB_18121411_205.jpg

GTX88437 WB Image

WB analysis of pig skeletal muscle (A) and heart (B) lysates using GTX88437 Caveolin 3 antibody, N-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88437_2329_WB_18121411_723.jpg

GTX88437 WB Image

WB analysis of mouse (A) and rat (B) heart lysates using GTX88437 Caveolin 3 antibody, N-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88437_962_IHC-P_18121411_735.jpg

GTX88437 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX88437 Caveolin 3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329