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Caveolin 3 antibody [N1N2], N-term

KO/KD
Anti-Caveolin 3 antibody [N1N2], N-term used in Western Blot (WB). GTX109650
Anti-Caveolin 3 antibody [N1N2], N-term used in Western Blot (WB). GTX109650
Anti-Caveolin 3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109650
Anti-Caveolin 3 antibody [N1N2], N-term used in Western Blot (WB). GTX109650

Cat. No. GTX109650

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Caveolin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

mouse muscle , rat muscle

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949854

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caveolin 3 , LGMD1C , LQT9 , MPDT , RMD2 , VIP-21 , VIP21

Cellular Localization

Golgi apparatus membrane; Single-pass membrane protein , Cell membrane; Single-pass membrane protein , Membrane , caveola; Single-pass membrane protein , Golgi apparatus membrane , Cell membrane , Membrane , caveola

Background

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caveolin 3 antibody [N1N2], N-term used in Western Blot (WB). GTX109650

GTX109650 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Caveolin 3 antibody [N1N2], N-term (GTX109650) diluted at 1:1000.

Anti-Caveolin 3 antibody [N1N2], N-term used in Western Blot (WB). GTX109650

GTX109650 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 3 antibody [N1N2], N-term (GTX109650) diluted at 1:1000.

Anti-Caveolin 3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109650

GTX109650 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Caveolin-3(GTX109650) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Caveolin 3 antibody [N1N2], N-term used in Western Blot (WB). GTX109650

GTX109650 WB Image

KO/KD

The data was published in the journal Hum Mutat in 2011. PMID: 21294223

REFERENCE

Application Reference

Bohm J et al. Brain 2014; 137 (Pt 12) : 3160-70 Adult-onset autosomal dominant centronuclear myopathy due to BIN1 mutations.
Application : WB
Reactivity : Human
Ullrich ND et al. Hum Mutat 2011; 32 (3) : 309-17 Alterations of excitation-contraction coupling and excitation coupled Ca(2+) entry in human myotubes carrying CAV3 mutations linked to rippling muscle.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169