APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.72 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Cbl-b. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Cbl proto-oncogene B , Cbl-b , Nbla00127 , RNF56
Cellular Localization
Cytoplasm
Background
E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.
Database
Research Area
DATA IMAGES
![Anti-Cbl-b antibody [C2C3], C-term used in Western Blot (WB). GTX102389](/upload/website/prouct_img/middle/GTX102389/GTX102389_WB_19011506_911.jpg "Anti-Cbl-b antibody [C2C3], C-term used in Western Blot (WB). GTX102389")
|
GTX102389 WB Image
Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX102389 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Cbl-b antibody [C2C3], C-term (GTX102389). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cbl-b antibody [C2C3], C-term (GTX102389). Be the first to share your experience with this product.
![Anti-Cbl-b antibody [C2C3], C-term used in Western Blot (WB). GTX102389](/upload/website/prouct_img/small/GTX102389/GTX102389_WB_19011506_911.jpg "Anti-Cbl-b antibody [C2C3], C-term used in Western Blot (WB). GTX102389")
![Anti-Cbl-b antibody [C2C3], C-term used in Western Blot (WB). GTX102389](/upload/website/prouct_img/normal/GTX102389/GTX102389_WB_19011506_911.jpg "Anti-Cbl-b antibody [C2C3], C-term used in Western Blot (WB). GTX102389")
