Western blot analysis with Cdc22 antibody. Cdc22 protein (92 kDa) was detected by western blotting in S. pombe crude extract. 7.5 % SDS-PAGE was used. Anti-Cdc22 antibody was used at 1/1,000 dilution and and secondary antibody, anti-rabbit IgG conjugated with HRP, was used at 1/10,000 ditluion.