Identification of endogenous Cdc48 protein in crude extract of S. cerevisiae Total protein (40 μg) of S. cerevisiae strain W303 was analyzed by western blot. 1. Anti-Cdc48 antibody at 1/500. 2. Anti-Cdc48 antibody at 1/2,000 3. Anti-Cdc48 antibody at 1/5,000 As second antibody, HRP-conjugated anti-rabbit IgG was used at 1/10,000 dilution. Positions of molecular size markers were shown in kDa on the left. Cdc48 calculated from amino acids sequence is 92 kDa