GeneTex
United States (US)

Cdh1 antibody

GTX125890_42508_20160523_IHC-Wm_Z_18121410_308.jpg
GTX125890_40527_IHC_Z_18121410_274.jpg

Cat No. GTX125890

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-Fr, IHC-Wm, IHC
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

Zebrafish embryo
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Cdh1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cadherin 1, Type 1, E-Cadherin (Epithelial),E-Cadherin,Cdh1

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Research Area

DATA IMAGES
GTX125890_42508_20160523_IHC-Wm_Z_18121410_308.jpg

GTX125890 IHC-Wm Image

Cdh1 antibody detects Cdh1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Cdh1 antibody (GTX125890) dilution: 1:50.

GTX125890_40527_IHC_Z_18121410_274.jpg

GTX125890 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using E-cadherin (GTX125890) antibody at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199