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Cdh1 antibody

Anti-Cdh1 antibody used in IHC (Whole mount) (IHC-Wm). GTX125890
Anti-Cdh1 antibody used in Immunohistochemistry (IHC). GTX125890

Cat. No. GTX125890

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Cdh1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
IHC 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (80%)| Rat (83%)| Bovine (82%)| Dog (80%)| Pig (83%)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Cdh1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167551

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 1, type 1, E-cadherin (epithelial) , E-cadherin

Database

Research Area

DATA IMAGES

Anti-Cdh1 antibody used in IHC (Whole mount) (IHC-Wm). GTX125890

GTX125890 IHC-Wm Image

Cdh1 antibody detects Cdh1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Cdh1 antibody (GTX125890) dilution: 1:50.
Anti-Cdh1 antibody used in Immunohistochemistry (IHC). GTX125890

GTX125890 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using E-cadherin (GTX125890) antibody at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

REFERENCE

Application Reference

Lee JG et al. Microbiome 2022; 10 (1) : 3 Host tp53 mutation induces gut dysbiosis eliciting inflammation through disturbed sialic acid metabolism.
Application : IHC-Fr
Reactivity : Zebrafish
Klatt Shaw D et al. Dev Cell 2021; 56 (5) : 613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.
Application : IHC-Fr
Reactivity : Zebrafish
Liu R et al. Cell Transplant 2020; 29 : 963689720969167 Characteristics of TGFBR1-EGFR-CTNNB1-CDH1 Signaling Axis in Wnt-Regulated Invasion and Migration in Lung Cancer.
Liu J et al. PLoS Biol 2019; 17 (8) : e3000203 Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.
Application : IHC-Wm
Reactivity : Zebrafish
Paul CD et al. Cell Syst 2019; 9 (2) : 187-206.e16 Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
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REVIEW

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Application Reference
Lee JG et al. Microbiome 2022; 10 (1):3 Host tp53 mutation induces gut dysbiosis eliciting inflammation through disturbed sialic acid metabolism.
Application : IHC-Fr
Reactivity : Zebrafish
Klatt Shaw D et al. Dev Cell 2021; 56 (5):613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.
Application : IHC-Fr
Reactivity : Zebrafish
Liu R et al. Cell Transplant 2020; 29:963689720969167 Characteristics of TGFBR1-EGFR-CTNNB1-CDH1 Signaling Axis in Wnt-Regulated Invasion and Migration in Lung Cancer.
Liu J et al. PLoS Biol 2019; 17 (8):e3000203 Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.
Application : IHC-Wm
Reactivity : Zebrafish
Paul CD et al. Cell Syst 2019; 9 (2):187-206.e16 Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Enya S et al. Dis Model Mech 2018; 11 (8) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.
Hehr CL et al. Cell Adh Migr 2018; 12 (5):489-502 Polarity and morphogenesis of the eye epithelium requires the adhesion junction associated adaptor protein Traf4.
Warga RM et al. Genetics 2018; 210 (4):1391-1409 Probing Cadherin Interactions in Zebrafish with E- and N-Cadherin Missense Mutants.
Application : IHC-Wm
Reactivity : Zebrafish
Mochizuki T et al. Development 2018; 145 (20) Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Franco J.J. et al. bioRxiv 2018; (Epub) Cellular Crowding Influences Extrusion and Proliferation to Facilitate Epithelial Tissue Repair.
Application : IHC-Wm
Reactivity : Zebrafish
Enya S et al. bioRxiv 2017; (Epub) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in tumor's adverse effects on host.
Application : IHC-Fr
Reactivity : Zebrafish
Jimenez L et al. Dis Model Mech 2016; 9 (4):389-400 Phenotypic chemical screening using a zebrafish neural crest EMT reporter identifies retinoic acid as an inhibitor of epithelial morphogenesis.
Application : IHC-Wm
Reactivity : Zebrafish
Ma WR et al. Development 2015; 142 (4):763-73 Jag1b is essential for patterning inner ear sensory cristae by regulating anterior morphogenetic tissue separation and preventing posterior cell death.
Application : IHC-Wm
Reactivity : Zebrafish
Chou CW et al. PLoS One 2014; 9 (9):e107997 The hemodynamically-regulated vascular microenvironment promotes migration of the steroidogenic tissue during its interaction with chromaffin cells in the zebrafish embryo.
Application : IHC-Wm
Reactivity : Zebrafish
Xiong F et al. Cell 2014; 159 (2):415-27 Interplay of cell shape and division orientation promotes robust morphogenesis of developing epithelia.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169