Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cdh2 antibody [HL2496] (GTX638854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cdh2 antibody [HL2496] detects Cdh2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Cdh2 stained by Cdh2 antibody [HL2496] (GTX638854) diluted at 1:50.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC