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Cdh2 antibody

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125885
Anti-Cdh2 antibody used in Western Blot (WB). GTX125885
Anti-Cdh2 antibody used in Western Blot (WB). GTX125885

Cat. No. GTX125885

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Cdh2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

zebrafish brain

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Cdh2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885609

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 2, type 1, N-cadherin (neuronal) , bib , glo , lyr , ncad , pac , wu:fb47h04

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125885

GTX125885 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein on cell membranes by immunohistochemical analysis Sample: Agarose-embedded zebrafish embryo Cdh2 antibody (GTX125885) dilution: 1:200. Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.

Anti-Cdh2 antibody used in Western Blot (WB). GTX125885

GTX125885 WB Image

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cdh2 antibody (GTX125885) diluted at 1:1000.

Anti-Cdh2 antibody used in Western Blot (WB). GTX125885

GTX125885 WB Image

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cdh2 antibody (GTX125885) diluted at 1:2000.

REFERENCE

Application Reference

Klatt Shaw D et al. Dev Cell 2021; 56 (5) : 613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.
Application : IHC-Fr
Reactivity : Zebrafish
Ahsan K et al. Dev Biol 2019; 448 (1) : 16-35 Prickle1 is required for EMT and migration of zebrafish cranial neural crest.
Huang M et al. Sci Total Environ 2019; 656 : 1337-1345 Exposure to acrylamide disrupts cardiomyocyte interactions during ventricular morphogenesis in zebrafish embryos.
Application : IHC-Wm
Reactivity : Zebrafish
Powell D et al. Sci Rep 2018; 8 (1) : 13285 Cxcr1 mediates recruitment of neutrophils and supports proliferation of tumor-initiating astrocytes in vivo.
Application : IHC-Wm
Reactivity : Zebrafish
Gays D et al. Development 2017; 144 (3) : 464-478 An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates.
Application : WB
Reactivity : Zebrafish
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169