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Cdh2 antibody

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125885
Anti-Cdh2 antibody used in Western Blot (WB). GTX125885
Anti-Cdh2 antibody used in Western Blot (WB). GTX125885

Cat. No. GTX125885

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Cdh2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

zebrafish brain

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Cdh2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885609

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 2, type 1, N-cadherin (neuronal) , bib , glo , lyr , ncad , pac , wu:fb47h04

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-Cdh2 antibody used in IHC (Whole mount) (IHC-Wm). GTX125885

GTX125885 IHC-Wm Image

Cdh2 antibody detects Cdh2 protein on cell membranes by immunohistochemical analysis Sample: Agarose-embedded zebrafish embryo Cdh2 antibody (GTX125885) dilution: 1:200. Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.

Anti-Cdh2 antibody used in Western Blot (WB). GTX125885

GTX125885 WB Image

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cdh2 antibody (GTX125885) diluted at 1:2000.

Anti-Cdh2 antibody used in Western Blot (WB). GTX125885

GTX125885 WB Image

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cdh2 antibody (GTX125885) diluted at 1:1000.

REFERENCE

Application Reference

Klatt Shaw D et al. Dev Cell 2021; 56 (5) : 613-626.e7 Localized EMT reprograms glial progenitors to promote spinal cord repair.
Application : IHC-Fr
Reactivity : Zebrafish
Ahsan K et al. Dev Biol 2019; 448 (1) : 16-35 Prickle1 is required for EMT and migration of zebrafish cranial neural crest.
Huang M et al. Sci Total Environ 2019; 656 : 1337-1345 Exposure to acrylamide disrupts cardiomyocyte interactions during ventricular morphogenesis in zebrafish embryos.
Application : IHC-Wm
Reactivity : Zebrafish
Powell D et al. Sci Rep 2018; 8 (1) : 13285 Cxcr1 mediates recruitment of neutrophils and supports proliferation of tumor-initiating astrocytes in vivo.
Application : IHC-Wm
Reactivity : Zebrafish
Gays D et al. Development 2017; 144 (3) : 464-478 An exclusive cellular and molecular network governs intestinal smooth muscle cell differentiation in vertebrates.
Application : WB
Reactivity : Zebrafish
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169